November 6, 2018
Structure and Activity of Lipid Bilayer within a Membrane Protein Transporter
Weihua Qiu, Ziao Fu, Guoyan G. Xu, Robert A. Grassucci, Yan Zhang, Joachim Frank, Wayne A. Hendrickson, Youzhong Guo
Membrane proteins function in native cell membranes, but extraction into isolated particles is needed for many biochemical and structural analyses. Commonly used detergent-extraction methods destroy naturally associated lipid bilayers. Here, we devised a detergent-free method for preparing cell membrane nanoparticles to study the multidrug...