October 6, 2015
Two promising future developments of cryo-EM: capturing short-lived states and mapping a continuum of states of a macromolecule.
Bo Chen and Joachim Frank
The capabilities and application range of cryogenic electron microscopy (cryo-EM) method have expanded vastly in the last two years, thanks to the advances provided by direct detection devices and computational classification tools. We take this review as an opportunity to sketch out promising developments of cryo-EM in two important directions: (1) imaging of short-lived states...