Until late last year, an amber-colored structure stood on a window sill in Joachim Frank’s lab, where it glowed, backlit by the late morning sun. You would be forgiven for thinking that this series of oddly shaped plastic contours,...
Read MoreNew Insights into Ribosome Structure and Function Amy Jobe, Zheng Liu, Cristina Gutierrez-Vargas, and Joachim Frank In the past 4 years, because of the advent of new cameras, many ribosome structures have been solved by cryoelectron microscopy (cryo-EM) at high, often near-atomic resolution, bringing new mechanistic insights into the processes of translation initiation, peptide elongation, termination,...Posted by: Joachim Frank | May 4, 2018 | No comment
Read MoreCryo-EM shows stages of initial codon selection on the ribosome by aa-tRNA in ternary complex with GTP and the GTPase-deficient EF-TuH84A Marcus Fislage, Jingji Zhang, Zuben Patrick Brown, Chandra Sekhar Mandava, Suparna Sanya, Mans Ehrenberg and Joachim Frank ABSTRACT The GTPase EF-Tu in ternary complex with GTP and aminoacyl-tRNA (aa-tRNA) promotes rapid and accurate delivery of cognate aa-tRNAs to the ribosomal...Posted by: Joachim Frank | May 1, 2018 | No comment
Read MoreNew Opportunities Created by Single-Particle Cryo-EM: The Mapping of Conformational Space is now published online at http://pubs.acs.org/doi/full/Posted by: Joachim Frank | April 30, 2018 | No comment
10.1021/acs.biochem.8b00064 and is available open access as part of the ACS Editors' Choice initiative. ACS Editors’ ChoiceOne...
Read MoreIdentification of ions in experimental electrostatic potential maps. Jimin Wang, Zheng Liu, Joachim Frank and Peter Moore. International Union of Crystallography Journal ABSTRACT Cryo-electron microscopy (EM) directly images the distribution of electrostatic potential within macromolecules, and thus can provide much more information about atomic charges than X-ray crystallography normally does. The electron scattering length of an isolated ion is quite different from that of the corresponding neutral atom. The difference is very large at small scattering angles where the effects of electron...Posted by: Joachim Frank | April 19, 2018 | No comment
Read Morehttps://www.worldscientific.com/worldscibooks/10.1142/10844 Series in Structural Biology: Volume 10 Single-Particle Cryo-Electron Microscopy The Path Toward Atomic Resolution/ Selected Papers...Posted by: Joachim Frank | April 16, 2018 | No comment
Read MoreFrank Lab's undergraduate researcher, Binita Shah, was filmed in Columbia Science Review'sPosted by: Joachim Frank | April 9, 2018 | No comment
"Scientists in the Making" series.
Read MoreGünter Blobel was a German biologist born in Silesia, which is now part of Poland. He received the 1999 Nobel Prize in Physiology and Medicine for the discovery that proteins have intrinsic signals – Zip code-like -- that govern their transport and localization in the cell. Following his pioneering biochemical discoveries on signaling he postulated the existence of a “translocon,” a pore in the ER membrane specialized to receive...Posted by: Joachim Frank | February 20, 2018 | No comment
Read MoreDonald Parsons passed away earlier this month at age 89. He established high-voltage electron microscopy at the Division of Labs and Research of the New York State Department of Health in Albany, later to be renamed Wadsworth Center. Parsons was convinced that most cancers were caused by viruses, and that the reason they had not been found was a sampling problem. Imaging whole cells in 3D with 1MV electrons would be a way to find...Posted by: Joachim Frank | December 31, 2017 | No comment