Structure of human GABAB receptor in an inactive state
Jinseo Park, Ziao Fu, Aurel Frangaj, Jonathan Liu, Lidia Mosyak, Tong Shen, Vesna N. Slavkovich, Kimberly M. Ray, Jaume Taura, Baohua Cao, Yong Geng, Hao Zuo, Yongjun Kou, Robert Grassucci, Shaoxia Chen, Zheng Liu, Xin Lin, Justin P. Williams, William J. Rice, Edward T. Eng, Rick K. Huang, Rajesh K. Soni, Brian Kloss, Zhiheng Yu, Jonathan A. Javitch, Wayne A. Hendrickson, Paul A. Slesinger, Matthias Quick, Joseph Graziano, Hongtao Yu, Oliver Fiehn, Oliver B. Clarke, Joachim Frank &
Qing R. Fan
I’m proud of being part of this study as it demonstrates highly successful transfer of technology from my lab to Qing’s at Columbia University:
Qing Fan, a former postdoc of Wayne Hendrickson, is an associate professor with experience in X-ray crystallography. Ziao (Jack) Fu, who was a graduate student in my group until a year ago (now postdoc at Rockefeller) trained the third author Aurel Frangaj in every aspect of cryo-EM and the two managed to get the first reconstructions at about 5 Å resolution after a 2-year effort. Assistant professor Oliver Clarke, also one of Wayne’s former postdocs, who got his cryo-EM training earlier from my former postdoc Amedee des Georges (now at CUNY) in the Ryanodine receptor project, then applied the most recent tools of refinement and drive the resolution up to the range of 3.3 Å that is sufficient for modeling the molecule.